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CHatgpt summary & Analysis
Abstract
A transgenic soybean plant resistant to soybean cyst nematode (SCN), or parts thereof, are provided. Also provided are methods of increasing SCN resistance of a soybean plant and associated DNA constructs.
Claims
A plant of an agronomically elite soybean variety, comprising a first polynucleotide encoding a serine hydroxymethyltransferase promoter that functions in the soybean plant operably linked to a second polynucleotide encoding a polypeptide having serine hydroxymethyltransferase activity;
wherein said first polynucleotide comprises SEQ ID NO: 1, or a sequence at least 95% identical thereto, or a full-length complement thereof, or a functional fragment thereof;
wherein said first polynucleotide further comprises one or more mutations of SEQ ID NO: 1 selected from the group consisting of: A3959T, G3726C, A3444T, C3147T, A3130C, T3037C, G2999C, C2998T, T2979C, C2846T, G2475T, A2420G, C2416T, +2323T, T2051A, G2050C, A1606G, T1523-, G1164A, T1156A, A403C, C380T, A338T, T329A, T313C, T225G, T225-, A133G, A133-, G28T, and G28-; and
wherein the plant has increased soybean cyst nematode (SCN) resistance compared to a control soybean plant lacking said first polynucleotide.
The plant of claim 1, wherein said polypeptide having serine hydroxymethyltransferase activity comprises SEQ ID NO: 2, or a sequence at least 95% identical thereto, or a full-length complement thereof, or a functional fragment thereof, and wherein said polypeptide having serine hydroxymethyltransferase activity further comprises one or more mutations of SEQ ID NO: 2 selected from the group consisting of: I107F, P200R, P200-, N459Y, and N459H.
The plant of claim 2, wherein said second polynucleotide has increased expression, an altered expression pattern, or an increased copy number.
The plant of claim 3, wherein said second polynucleotide has a copy number of at least 2.
The plant of claim 1, further comprising a third polynucleotide encoding an alpha soluble NSF attachment protein promoter that functions in the soybean plant operably linked to a fourth polynucleotide encoding a polypeptide having alpha soluble NSF attachment protein activity;
wherein said third polynucleotide comprises SEQ ID NO: 3, or a sequence at least 95% identical thereto, or a full-length complement thereof, or a functional fragment thereof; and
wherein said third polynucleotide further comprises one or more mutations of SEQ ID NO: 3 selected from the group consisting of: C1161A, C1082A, C1044A, C1025T, A1016C, T997A, C970A, C970-, G829T, G825T, A815C, A363T, T336C, G334A, T328C, T327A, C267G, T157G, T83A, C57T, and T36A.
The plant of claim 5, wherein said polypeptide having alpha soluble NSF attachment protein activity comprises SEQ ID NO: 4, or a sequence at least 95% identical thereto, or a full-length complement thereof, or a functional fragment thereof, and wherein said polypeptide having alpha soluble NSF attachment protein activity further comprises one or more mutations of SEQ ID NO: 4 selected from the group consisting of: A111D, Q203K, D208E, I238V, E285Q, D286Y, D286H, D287E, +287A, +287V, L288I, and +288T.
The plant of claim 6, wherein said fourth polynucleotide has increased expression, an altered expression pattern, or an increased copy number.
The plant of claim 7, wherein said fourth polynucleotide has a copy number of at least 2.
The plant of claim 3, further comprising a third polynucleotide encoding an alpha soluble NSF attachment protein promoter that functions in the soybean plant operably linked to a fourth polynucleotide encoding a polypeptide having alpha soluble NSF attachment protein activity;
wherein said third polynucleotide comprises SEQ ID NO: 3, or a sequence at least 95% identical thereto, or a full-length complement thereof, or a functional fragment thereof; and
wherein said third polynucleotide further comprises one or more mutations of SEQ ID NO: 3 selected from the group consisting of: C1161A, C1082A, C1044A, C1025T, A1016C, T997A, C970A, C970-, G829T, G825T, A815C, A363T, T336C, G334A, T328C, T327A, C267G, T157G, T83A, C57T, and T36A.
The plant of claim 9, wherein said polypeptide having alpha soluble NSF attachment protein activity comprises SEQ ID NO: 4, or a sequence at least 95% identical thereto, or a full-length complement thereof, or a functional fragment thereof, and wherein said polypeptide having alpha soluble NSF attachment protein activity further comprises one or more mutations of SEQ ID NO: 4 selected from the group consisting of: A111D, Q203K, D208E, I238V, E285Q, D286Y, D286H, D287E, +287A, +287V, L288I, and +288T.
The plant of claim 10, wherein said fourth polynucleotide has increased expression, an altered expression pattern, or an increased copy number.
The plant of claim 11, wherein said fourth polynucleotide has a copy number of at least 2.
A plant part of the plant of claim 1.
A plant of an agronomically elite soybean variety, comprising a first polynucleotide encoding a serine hydroxymethyltransferase promoter that functions in the soybean plant operably linked to a second polynucleotide encoding a polypeptide having serine hydroxymethyltransferase activity;
wherein said polypeptide having serine hydroxymethyltransferase activity comprises SEQ ID NO: 2, or a sequence at least 95% identical thereto, or a full-length complement thereof, or a functional fragment thereof;
wherein said polypeptide having serine hydroxymethyltransferase activity further comprises one or more mutations of SEQ ID NO: 2 selected from the group consisting of: I107F, P200R, P200-, N459Y, and N459H;
wherein the plant has increased soybean cyst nematode (SCN) resistance compared to a control soybean plant lacking said second polynucleotide; and
wherein said second polynucleotide has increased expression, an altered expression pattern, or an increased copy number.
The plant of claim 14, wherein said second polynucleotide has a copy number of at least 2.
The plant of claim 14, further comprising a third polynucleotide encoding an alpha soluble NSF attachment protein promoter that functions in soybean operably linked to a fourth polynucleotide encoding a polypeptide having alpha soluble NSF attachment protein activity;
wherein said third polynucleotide comprises SEQ ID NO: 3, or a sequence at least 95% identical thereto, or a full-length complement thereof, or a functional fragment thereof; and
wherein said third polynucleotide further comprises one or more mutations of SEQ ID NO: 3 selected from the group consisting of: C1161A, C1082A, C1044A, C1025T, A1016C, T997A, C970A, C970-, G829T, G825T, A815C, A363T, T336C, G334A, T328C, T327A, C267G, T157G, T83A, C57T, and T36A.
The plant of claim 16, wherein said polypeptide having alpha soluble NSF attachment protein activity comprises SEQ ID NO: 4, or a sequence at least 95% identical thereto, or a full-length complement thereof, or a functional fragment thereof, and wherein said polypeptide having alpha soluble NSH attachment protein activity further comprises one or more mutations of SEQ ID NO: 4 selected from the group consisting of: A111D, Q203K, D208E, I238V, E285Q, D286Y, D286H, D287E, +287A, +287V, L288I, and +288T.
The plant of claim 17, wherein said fourth polynucleotide has increased expression, an altered expression pattern, or an increased copy number.
The plant of claim 18, wherein said fourth polynucleotide has a copy number of at least 2.
A plant part of the plant of claim 14.
A DNA construct comprising a first polynucleotide encoding a serine hydroxymethyltransferase promoter that functions in a soybean plant operably linked to a second polynucleotide encoding a polypeptide having serine hydroxymethyltransferase activity;
wherein said first polynucleotide comprises SEQ ID NO: 1, or a sequence at least 95% identical thereto, or a full-length complement thereof, or a functional fragment thereof; and
wherein said first polynucleotide further comprises one or more mutations of SEQ ID NO: 1 selected from the group consisting of: A3959T, G3726C, A3444T, C3147T, A3130C, T3037C, G2999C, C2998T, T2979C, C2846T, G2475T, A2420G, C2416T, +2323T, T2051A, G2050C, A1606G, T1523-, G1164A, T1156A, A403C, C380T, A338T, T329A, T313C, T225G, T225-, A133G, A133-, G28T, and G28-.
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